Background: Vector incrimination is of utmost importance in the transmission of Leishmania species identified in the Volta region of Ghana. The feeding preference of sand flies provides valuable information about the vector-host interactions, including reservoir host. Objective: The main aim of this study was to identify the blood meal in collected sand flies and its source from three villages in a cutaneous leishmaniasis endemic area in the Ho Municipality. Method: Sand flies was collected using CDC light straps and sticky paper traps from Lume Atsiame, Dodome Dogblome and Dodome Awuiasu. They were morphologically identified by taxonomic keys. DNA was extracted from the individual sand flies using the potassium acetate extraction method. Polymerase chain reaction (PCR) amplification of cytochrome b gene (cyt b) fragment was carried out and subsequently DNA sequenced. Results: Of a total of three hundred and sixty-three (363) female sand flies that were analysed, eighty-four (84) representing 23.14% were positive for blood meal presence with an amplified DNA of the expected size, 359bp. Out of the number that was sequenced, the cyt b sequencing revealed that four (4) blood fed females fed on bloodmeal from human (Homo sapiens) and house mouse (Mus musculus). Conclusion: The blood meal presence was more predominant in Sergentomyia ghesquierei and least in Sergentomyia schwetzi. This may aid in the development of effective strategies to control cutaneous leishmaniasis in the endemic areas.
| Published in | American Journal of Biomedical and Life Sciences (Volume 8, Issue 4) |
| DOI | 10.11648/j.ajbls.20200804.12 |
| Page(s) | 69-75 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
Cytochrome B Gene, Feeding Preferences, Identification, Polymerase Chain Reaction, Sequencing
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APA Style
Edna Dzifa Doe, Godwin Kwakye-Nuako, Alexander Egyir-Yawson. (2020). Identification of Blood Meal of Sand Flies in a Cutaneous Leishmaniasis Endemic Area, Volta Region-Ghana. American Journal of Biomedical and Life Sciences, 8(4), 69-75. https://doi.org/10.11648/j.ajbls.20200804.12
ACS Style
Edna Dzifa Doe; Godwin Kwakye-Nuako; Alexander Egyir-Yawson. Identification of Blood Meal of Sand Flies in a Cutaneous Leishmaniasis Endemic Area, Volta Region-Ghana. Am. J. Biomed. Life Sci. 2020, 8(4), 69-75. doi: 10.11648/j.ajbls.20200804.12
AMA Style
Edna Dzifa Doe, Godwin Kwakye-Nuako, Alexander Egyir-Yawson. Identification of Blood Meal of Sand Flies in a Cutaneous Leishmaniasis Endemic Area, Volta Region-Ghana. Am J Biomed Life Sci. 2020;8(4):69-75. doi: 10.11648/j.ajbls.20200804.12
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Download@article{10.11648/j.ajbls.20200804.12,
author = {Edna Dzifa Doe and Godwin Kwakye-Nuako and Alexander Egyir-Yawson},
title = {Identification of Blood Meal of Sand Flies in a Cutaneous Leishmaniasis Endemic Area, Volta Region-Ghana},
journal = {American Journal of Biomedical and Life Sciences},
volume = {8},
number = {4},
pages = {69-75},
doi = {10.11648/j.ajbls.20200804.12},
url = {https://doi.org/10.11648/j.ajbls.20200804.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20200804.12},
abstract = {Background: Vector incrimination is of utmost importance in the transmission of Leishmania species identified in the Volta region of Ghana. The feeding preference of sand flies provides valuable information about the vector-host interactions, including reservoir host. Objective: The main aim of this study was to identify the blood meal in collected sand flies and its source from three villages in a cutaneous leishmaniasis endemic area in the Ho Municipality. Method: Sand flies was collected using CDC light straps and sticky paper traps from Lume Atsiame, Dodome Dogblome and Dodome Awuiasu. They were morphologically identified by taxonomic keys. DNA was extracted from the individual sand flies using the potassium acetate extraction method. Polymerase chain reaction (PCR) amplification of cytochrome b gene (cyt b) fragment was carried out and subsequently DNA sequenced. Results: Of a total of three hundred and sixty-three (363) female sand flies that were analysed, eighty-four (84) representing 23.14% were positive for blood meal presence with an amplified DNA of the expected size, 359bp. Out of the number that was sequenced, the cyt b sequencing revealed that four (4) blood fed females fed on bloodmeal from human (Homo sapiens) and house mouse (Mus musculus). Conclusion: The blood meal presence was more predominant in Sergentomyia ghesquierei and least in Sergentomyia schwetzi. This may aid in the development of effective strategies to control cutaneous leishmaniasis in the endemic areas.},
year = {2020}
}
TY - JOUR T1 - Identification of Blood Meal of Sand Flies in a Cutaneous Leishmaniasis Endemic Area, Volta Region-Ghana AU - Edna Dzifa Doe AU - Godwin Kwakye-Nuako AU - Alexander Egyir-Yawson Y1 - 2020/06/20 PY - 2020 N1 - https://doi.org/10.11648/j.ajbls.20200804.12 DO - 10.11648/j.ajbls.20200804.12 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 69 EP - 75 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20200804.12 AB - Background: Vector incrimination is of utmost importance in the transmission of Leishmania species identified in the Volta region of Ghana. The feeding preference of sand flies provides valuable information about the vector-host interactions, including reservoir host. Objective: The main aim of this study was to identify the blood meal in collected sand flies and its source from three villages in a cutaneous leishmaniasis endemic area in the Ho Municipality. Method: Sand flies was collected using CDC light straps and sticky paper traps from Lume Atsiame, Dodome Dogblome and Dodome Awuiasu. They were morphologically identified by taxonomic keys. DNA was extracted from the individual sand flies using the potassium acetate extraction method. Polymerase chain reaction (PCR) amplification of cytochrome b gene (cyt b) fragment was carried out and subsequently DNA sequenced. Results: Of a total of three hundred and sixty-three (363) female sand flies that were analysed, eighty-four (84) representing 23.14% were positive for blood meal presence with an amplified DNA of the expected size, 359bp. Out of the number that was sequenced, the cyt b sequencing revealed that four (4) blood fed females fed on bloodmeal from human (Homo sapiens) and house mouse (Mus musculus). Conclusion: The blood meal presence was more predominant in Sergentomyia ghesquierei and least in Sergentomyia schwetzi. This may aid in the development of effective strategies to control cutaneous leishmaniasis in the endemic areas. VL - 8 IS - 4 ER -
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