Medicinal plants are increasingly emerging as a real alternative that can contribute to the fight against cancer. The purpose of this study was to evaluate in vitro the antiradical, anti-lipoxygenase and cytotoxic properties on cancerous cells in culture of the medicinal recipe «Acti-plus». The extraction of «Acti-plus» was carried out according the instructions of the traditional health practitioner. Phytochemical screening, as well as the determination of polyphenols and total flavonoids were performed using characterization and assay tests by spectrometry as well as by high performance thin layer chromatography (HPTLC). Antiradical tests for trapping DPPH and ABTS radicals were used to measure the antioxidant potential. Anti-lipoxygenase activity was studied using 15-lipoxygenase (15-LOX) inhibition test. The cytotoxic activity of aqueous extract of «Acti-plus» was evaluated in vitro on human cancers cell using MTT test. The results of the study showed that aqueous extract of «Acti-plus» contains flavonoids, coumarins and tannins. Total phenolic compounds (TPC) and total flavonoids (TFC) were 61.95 ± 0.01 mg GAE/g dry extract and 43.01 ± 0.05 mg QE/g dry extract respectively. The extract exhibited excellent scavenging capacity of DPPH (IC50 = 9.33 ± 1.53 µg/mL) and ABTS (IC50 = 53.33 ± 0.89 µg/mL) radicals antioxidant potential. The study also found that the extract moderately inhibits 15-lipoxygenase at 100 µg/mL. Aqueous extract of «Acti-plus» inhibited in a concentration-dependent manner the proliferation of the three cell lines with best activity (IC50 = 0.317 ± 0.02 mg/mL) on prostate cancer PC-3 cells. These properties give a rational basis for the use of the recipe «Acti-plus» in traditional medicine in Burkina Faso.
| Published in | Journal of Diseases and Medicinal Plants (Volume 9, Issue 4) |
| DOI | 10.11648/j.jdmp.20230904.14 |
| Page(s) | 148-155 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Cytotoxic, Antiradical, Anti-lipoxygenase, Acti-plus
| [1] | Omosa LK, Midiwo JO, Masila VM, Gisacho BM, Munayi R, Francisca-Kamakama, Chemutai KP, Elhaboob G, Saeed MEM, Hamdoun S, Kuete V, Efferth T. Cytotoxicity of 91 Kenyan indigenous medicinal plants towards human CCRF-CEM leukemia cells. Journal of Ethnopharmacology. 2016, 179, 177–196. DOI: 10.1016/j.jep.2015.12.028. |
| [2] | Sawadogo W, Teiten M, Cerella C, Guissou I, Diederich M. Cytotoxic Effect and NF-κB Inhibition of Fractions from Lantana ukambensis (Verbenacea). Planta Med. 2013, 79(10), s-0033-1348721. DOI: 10.1055/s-0033-1348721. |
| [3] | Efferth T, Saeed MEM, Mirghani E, Alim A, Yassin Z, Saeed E, Khalid HE, Daak S. Integration of phytochemicals and phytotherapy into cancer precision medicine. Oncotarget. 2017, 8(30), 50284–50304. DOI: 10.18632/oncotarget.17466. |
| [4] | Cragg GM, Newman DJ. Natural products: A continuing source of novel drug leads. Biochimica et Biophysica Acta (BBA) - General Subjects. 2013, 1830(6), 3670–3695. DOI: 10.1016/j.bbagen.2013.02.008. |
| [5] | Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA A Cancer J Clin. 2021, 71(3), Article 3. DOI: 10.3322/caac.21660. |
| [6] | Globocan. Estimated number of deaths from 2020 to 2040, Both sexes, age [0-85+], All cancers, Africa. Disponible sur: https://gco.iarc.fr/tomorrow/en/dataviz/isotype?types=1&single_unit=50000&populations=903. [Accessed 17 October 2023]. |
| [7] | Ochwang’i DO, Kimwele CN, Oduma JA, Gathumbi PK, Kiama SG, Efferth T. Cytotoxic activity of medicinal plants of the Kakamega County (Kenya) against drug-sensitive and multidrug-resistant cancer cells. Journal of Ethnopharmacology. 2018, 215, 233–240. DOI: 10.1016/j.jep.2018.01.004. |
| [8] | Coulidiati TH. Burden of cancer and role of traditional medicine in Burkina Faso. International Journal of Complementary and Alternative Medicine. 2019, 12 (5), Article 5. DOI: 10.15406/ijcam.2019.12.00473. |
| [9] | Kola P, Manjula SN, Metowogo K, Madhunapantula SV, Eklu-Gadegbeku K. Four Togolese plant species exhibiting cytotoxicity and antitumor activities lightning polytherapy approach in cancer treatment. Heliyon. 2023, 9 (3), e13869. DOI: 10.1016/j.heliyon.2023.e13869. |
| [10] | Dolatyari A, Eyvazzadeh O, Nateghi L. Determination of Dunaliella Salina Phenolic Compounds in Laboratory Different Conditions. Chem Methodol, 2021, (Online First). DOI: 10.22034/chemm.2022.2.4. |
| [11] | Wendkouni LMEB-K, Boukaré K, Adjaratou C-C, Tata KT, Emmanuel AMT, Mariam N-T. Phytochemical and biological investigations of extracts from the roots of Cocos nucifera L. (Arecaceae) and Carica papaya L. (Caricaceae), two plants used in traditional medicine. Afr J Biochem Res, 2021, 15(2), 28–35. DOI: 10.5897/AJBR2020.1107. |
| [12] | Velázquez E, Tournier HA, Mordujovich De Buschiazzo P, Saavedra G, Schinella GR. Antioxidant activity of Paraguayan plant extracts. Fitoterapia, 2003, 74(1–2), 91–97. DOI: 10.1016/S0367-326X(02)00293-9. |
| [13] | Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 1999, 26(9–10), 1231–1237. DOI: 10.1016/S0891-5849(98)00315-3. |
| [14] | Malterud KE, Rydland KM. Inhibitors of 15-Lipoxygenase from Orange Peel. J Agric Food Chem, 2000, 48(11), 5576–5580. DOI: 10.1021/jf000613v. |
| [15] | Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983, 65(1–2), 55–63. DOI: 10.1016/0022-1759(83)90303-4. |
| [16] | Fridlender M, Kapulnik Y, Koltai H. Plant derived substances with anti-cancer activity: from folklore to practice. Front Plant Sci 2015, 6. DOI: 10.3389/fpls.2015.00799. |
| [17] | Organisation Ouest Africaine de la Santé (OOAS). Manuel de formation des tradipraticiens. 2013, pp. 64. |
| [18] | Alabi MA, Muthusamy A, Kabekkodu SP, Adebawo OO, Satyamoorthy K, Ajagun EJ. In vitro cytotoxicity of recipes derived from Nigerian medicinal plants (NMPs) on breast cancer cells. International Journal of Chemical Science. 2017, 1, 90–97. |
| [19] | Raja KS, Taip FS, Azmi MMZ, Shishir MRI. Effect of pre-treatment and different drying methods on the physicochemical properties of Carica papaya L. leaf powder. Journal of the Saudi Society of Agricultural Sciences. 2019, 18(2), 150–156. DOI: 10.1016/j.jssas.2017.04.001. |
| [20] | Nile SH, Keum YS, Nile AS, Jalde SS, Patel RV. Antioxidant, anti‐inflammatory, and enzyme inhibitory activity of natural plant flavonoids and their synthesized derivatives. J Biochem & Molecular Tox. 2018, 32 (1), e22002. DOI: 10.1002/jbt.22002. |
| [21] | Tuli HS, Garg VK, Bhushan S, Uttam V, Sharma U, Jain A. Natural flavonoids exhibit potent anticancer activity by targeting microRNAs in cancer: A signature step hinting towards clinical perfection. Translational Oncology. 2023, 27, 101596. DOI: 10.1016/j.tranon.2022.101596. |
| [22] | Cao-Ngoc P, Leclercq L, Rossi J-C, Hertzog J, Tixier A-S, Chemat F. Water-Based Extraction of Bioactive Principles from Blackcurrant Leaves and Chrysanthellum americanum: A Comparative Study. Foods. 2020, 9 (10), 1478. DOI: 10.3390/foods9101478. |
| [23] | Guenne S, Ouattara N, Hilou A, Millogo JF, Nacoulma OG. Antioxidant, Enzyme Inhibition Activities and Polyphenol Contents of Three Asteraceae Species Used In Burkina Faso Traditionally Medicine. International Journal of Pharmacy and Pharmaceutical Sciences. 2011, 3, 524–528. |
| [24] | Mevy JP, Bessiere JM, Dherbomez M. Composition, Antimicrobial and Antioxidant Activities of the Volatile Oil of Chrysanthellum americanum (Linn.) Vatke. Journal of Essential Oil-Bearing Plants. 2012, 15 (3), 489–496. DOI: 10.1080/0972060X.2012.10644077. |
| [25] | Ferrara L. Use of Chrysantellum americanum (L.) vatke as supplement. European Scientific Journal. 2013, 9(36), 7. |
| [26] | Aissani N, Albouchi F, Sebai H. Anticancer Effect in Human Glioblastoma and Antioxidant Activity of Petroselinum crispum L. Methanol Extract. Nutrition and Cancer. 2021, 73 (11–12), 2605–2613. DOI: 10.1080/01635581.2020.1842894. |
| [27] | Al Aboody MS. Cytotoxic, antioxidant, and antimicrobial activities of Celery (Apium graveolens L.). Bioinformation. 2021, 17 (1), 147–156. DOI: 10.6026/97320630017147. |
| [28] | Kooti W, Daraei N. A Review of the Antioxidant Activity of Celery (Apium graveolens L). J Evid Based Complementary Altern Med. 2017, 22 (4), 1029–1034. DOI: 10.1177/2156587217717415. |
| [29] | Mariod A, Matthus B, Hussein I. Antioxidant activities of extracts from Combretum hartmannianum and Guiera senegalensis on the oxidative stability of sunflower oil. Emir J Food Agric. 2006, 18(2), 20. DOI: 10.9755/ejfa.v12i1.5136. |
| [30] | Sombie PAED, Hilou A, Mounier C, Coulibaly AY, Kiendrebeo M, Millogo JF. Antioxidant and Anti-inflammatory Activities from Galls of Guiera senegalensis J.F. Gmel (Combretaceae). Research J of Medicinal Plant. 2011, 5(4), 448–461. DOI: 10.3923/rjmp.2011.448.461. |
| [31] | Aristide T, Sylvin O, Mohamed BB, Adama K, Innocent PG. Phytochemical analysis and ovicidal activity of Cassia sieberiana, Guiera senegalensis and Excoecaria grahamii extracts. Afr J Pharm Pharmacol. 2017, 11(44), 554–560. DOI: 10.5897/AJPP2017.4837. |
| [32] | Bayala B, Bassole IHN, Maqdasy S, Baron S, Simpore J, Lobaccaro J-MA. Cymbopogon citratus and Cymbopogon giganteus essential oils have cytotoxic effects on tumor cell cultures. Identification of citral as a new putative anti-proliferative molecule. Biochimie. 2018, 153, 162–170. DOI: 10.1016/j.biochi.2018.02.013. |
| [33] | Bayala B, Coulibaly LL, Djigma F, Bunay J, Yonli A, Traore L, Simpore J. Chemical Composition of Essential Oil of Cymbopogon schoenanthus (L.) Spreng from Burkina Faso, and Effects against Prostate and Cervical Cancer Cell Lines. Molecules. 2023, 28 (11), 4561. DOI: 10.3390/molecules28114561. |
| [34] | Tang EL, Rajarajeswaran J, Fung S, Kanthimathi M. Petroselinum crispum has antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells. J Sci Food Agric. 2015, 95 (13), 2763–2771. DOI: 10.1002/jsfa.7078. |
| [35] | Fejes S, Kéry A, Blázovics A, Lugasi A, Lemberkovics E, Petri G, Szöke E. Investigation of the in vitro antioxidant effect of Petroselinum crispum (Mill.) Nym. ex A. W. Hill. Acta Pharm Hung. 1998, 68 (3), 150–156. |
| [36] | Weng C-J, Yen G-C. Flavonoids, a ubiquitous dietary phenolic subclass, exert extensive in vitro anti-invasive and in vivo anti-metastatic activities. Cancer Metastasis Rev. 2012, 31 (1–2), 323–351. DOI: 10.1007/s10555-012-9347-y. |
| [37] | Köken T, Koca B, Özkurt M, Erkasap N, Kuş G, Karalar M. Apium graveolens Extract Inhibits Cell Proliferation and Expression of Vascular Endothelial Growth Factor and Induces Apoptosis in the Human Prostatic Carcinoma Cell Line LNCaP. Journal of Medicinal Food. 2016, 19 (12), 1166–1171. DOI: 10.1089/jmf.2016.0061. |
| [38] | Kouamé J, Gnoula C, Palé E, Bassolé H, Guissou IP, Simporé J, Nikiéma JB. Etude des propriétés cytotoxiques et anti-radicalaires d’extraitsde feuilles et de galles de Guiera senegalensis J. F. Gmel (Combretacae). Science et technique, Sciences de la santé. 2009, 32 (1 et 2). |
| [39] | Fiot J, Sanon S, Azas N, Mahiou V, Jansen O, Angenot L, et al. Phytochemical and pharmacological study of roots and leaves of Guiera senegalensis J. F. Gmel (Combretaceae). Journal of Ethnopharmacology. 2006, 106 (2), 173–178. DOI: 10.1016/j.jep.2005.12.030. |
APA Style
Donald Wilfried Adico, M., Bayala, B., Valérie Elvira Jean Télesphore Bazié, B., Traoré, L., Théophane Yonli, A., et al. (2023). Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso. Journal of Diseases and Medicinal Plants, 9(4), 148-155. https://doi.org/10.11648/j.jdmp.20230904.14
ACS Style
Donald Wilfried Adico, M.; Bayala, B.; Valérie Elvira Jean Télesphore Bazié, B.; Traoré, L.; Théophane Yonli, A., et al. Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso. J. Dis. Med. Plants 2023, 9(4), 148-155. doi: 10.11648/j.jdmp.20230904.14
AMA Style
Donald Wilfried Adico M, Bayala B, Valérie Elvira Jean Télesphore Bazié B, Traoré L, Théophane Yonli A, et al. Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso. J Dis Med Plants. 2023;9(4):148-155. doi: 10.11648/j.jdmp.20230904.14
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.jdmp.20230904.14,
author = {Marc Donald Wilfried Adico and Bagora Bayala and Bapio Valérie Elvira Jean Télesphore Bazié and Lassina Traoré and Albert Théophane Yonli and Jacques Simporé},
title = {Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso},
journal = {Journal of Diseases and Medicinal Plants},
volume = {9},
number = {4},
pages = {148-155},
doi = {10.11648/j.jdmp.20230904.14},
url = {https://doi.org/10.11648/j.jdmp.20230904.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230904.14},
abstract = {Medicinal plants are increasingly emerging as a real alternative that can contribute to the fight against cancer. The purpose of this study was to evaluate in vitro the antiradical, anti-lipoxygenase and cytotoxic properties on cancerous cells in culture of the medicinal recipe «Acti-plus». The extraction of «Acti-plus» was carried out according the instructions of the traditional health practitioner. Phytochemical screening, as well as the determination of polyphenols and total flavonoids were performed using characterization and assay tests by spectrometry as well as by high performance thin layer chromatography (HPTLC). Antiradical tests for trapping DPPH and ABTS radicals were used to measure the antioxidant potential. Anti-lipoxygenase activity was studied using 15-lipoxygenase (15-LOX) inhibition test. The cytotoxic activity of aqueous extract of «Acti-plus» was evaluated in vitro on human cancers cell using MTT test. The results of the study showed that aqueous extract of «Acti-plus» contains flavonoids, coumarins and tannins. Total phenolic compounds (TPC) and total flavonoids (TFC) were 61.95 ± 0.01 mg GAE/g dry extract and 43.01 ± 0.05 mg QE/g dry extract respectively. The extract exhibited excellent scavenging capacity of DPPH (IC50 = 9.33 ± 1.53 µg/mL) and ABTS (IC50 = 53.33 ± 0.89 µg/mL) radicals antioxidant potential. The study also found that the extract moderately inhibits 15-lipoxygenase at 100 µg/mL. Aqueous extract of «Acti-plus» inhibited in a concentration-dependent manner the proliferation of the three cell lines with best activity (IC50 = 0.317 ± 0.02 mg/mL) on prostate cancer PC-3 cells. These properties give a rational basis for the use of the recipe «Acti-plus» in traditional medicine in Burkina Faso.
},
year = {2023}
}
TY - JOUR T1 - Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso AU - Marc Donald Wilfried Adico AU - Bagora Bayala AU - Bapio Valérie Elvira Jean Télesphore Bazié AU - Lassina Traoré AU - Albert Théophane Yonli AU - Jacques Simporé Y1 - 2023/12/11 PY - 2023 N1 - https://doi.org/10.11648/j.jdmp.20230904.14 DO - 10.11648/j.jdmp.20230904.14 T2 - Journal of Diseases and Medicinal Plants JF - Journal of Diseases and Medicinal Plants JO - Journal of Diseases and Medicinal Plants SP - 148 EP - 155 PB - Science Publishing Group SN - 2469-8210 UR - https://doi.org/10.11648/j.jdmp.20230904.14 AB - Medicinal plants are increasingly emerging as a real alternative that can contribute to the fight against cancer. The purpose of this study was to evaluate in vitro the antiradical, anti-lipoxygenase and cytotoxic properties on cancerous cells in culture of the medicinal recipe «Acti-plus». The extraction of «Acti-plus» was carried out according the instructions of the traditional health practitioner. Phytochemical screening, as well as the determination of polyphenols and total flavonoids were performed using characterization and assay tests by spectrometry as well as by high performance thin layer chromatography (HPTLC). Antiradical tests for trapping DPPH and ABTS radicals were used to measure the antioxidant potential. Anti-lipoxygenase activity was studied using 15-lipoxygenase (15-LOX) inhibition test. The cytotoxic activity of aqueous extract of «Acti-plus» was evaluated in vitro on human cancers cell using MTT test. The results of the study showed that aqueous extract of «Acti-plus» contains flavonoids, coumarins and tannins. Total phenolic compounds (TPC) and total flavonoids (TFC) were 61.95 ± 0.01 mg GAE/g dry extract and 43.01 ± 0.05 mg QE/g dry extract respectively. The extract exhibited excellent scavenging capacity of DPPH (IC50 = 9.33 ± 1.53 µg/mL) and ABTS (IC50 = 53.33 ± 0.89 µg/mL) radicals antioxidant potential. The study also found that the extract moderately inhibits 15-lipoxygenase at 100 µg/mL. Aqueous extract of «Acti-plus» inhibited in a concentration-dependent manner the proliferation of the three cell lines with best activity (IC50 = 0.317 ± 0.02 mg/mL) on prostate cancer PC-3 cells. These properties give a rational basis for the use of the recipe «Acti-plus» in traditional medicine in Burkina Faso. VL - 9 IS - 4 ER -
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso; Higher Normal School (ENS), Koudougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
